Tags
#Fauci Did It, ACE-2, Baric, Boyd Yount, Chimeric virus, chimeric viruses, China, coronaviruses, COVID-19, Daszak, Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus, Duke university, Duke-NUS, EcoHealth Alliance, Fauci, gain of function research, jail Fauci, lying to Congress, Mazet, Menachery, NIH, perjury, PREDICT, Rand Paul, SARS, SARS-CoV infectious clone, UC Davis, UK, Ukraine, University of North Carolina Chapel Hill, US Senate hearing, USAID, WIV, Wuhan, Wuhan lab, Yount, Zhengli Shi
Further below is the paper that US Senator Rand Paul referenced yesterday:
“Dr. Paul Joins Martha MacCallum to Expose Dr Fauci’s Lies”, July 21, 2021. Rand Paul to file criminal referral re Fauci for perjury re gain-of-function research: https://youtu.be/ZY7UoRQaq2Q
Peter Daszak and the Wuhan lab also got USAID funding, via a UC Davis program run by J.K. Mazet. Daszak was born, raised and educated in the UK and his father was Ukrainian, who seems to have fought on the Nazi side in WWII. Why is the UK allowed to send us their con-artists, or anyone else, when many indigenous British Americans don’t have right of return home?
Notice that Duke University is also implicated through their Singapore branch, Duke-NUS. Rand Paul earned his MD at Duke Medical School.
The paper is entitled:
“Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus, 2017, By
Ben Hu 1, Lei-Ping Zeng 1, Xing-Lou Yang 1, Xing-Yi Ge 1, Wei Zhang 1, Bei Li 1, Jia-Zheng Xie 1, Xu-Rui Shen 1, Yun-Zhi Zhang 2,3, Ning Wang 1, Dong-Sheng Luo 1, Xiao-Shuang Zheng 1, Mei-Niang Wang 1, Peter Daszak 4, Lin-Fa Wang 5, Jie Cui 1*, Zheng-Li Shi 1*
1 CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China, 2 Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China, 3 Dali University, Dali, China, 4 EcoHealth Alliance, New York, New York, United States of America, 5 Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore
“Funding: This work was jointly funded by National Natural Science Foundation of China (81290341, 31621061) to ZLS, China Mega-Project for Infectious Disease (2014ZX10004001-003) to ZLS, Scientific and technological basis special project (2013FY113500) to YZZ and ZLS from the Ministry of Science and Technology of China, the Strategic Priority Research Program of the Chinese Academy of Sciences (XDPB0301) to ZLS, the National Institutes of Health (NIAID R01AI110964), the USAID Emerging Pandemic Threats (EPT) PREDICT program to PD and ZLS, CAS Pioneer Hundred Talents Program to JC, NRF-CRP grant (NRF-CRP10-2012-05) to LFW and WIV ªOne-Three-Fiveº Strategic Program (WIV-135-TP1) to JC and ZLS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript”.
Excerpt: “In this study, we confirmed the use of human ACE2 as receptor of two novel SARSr-CoVs by using chimeric viruses with the WIV1 backbone replaced with the S gene of the newly identified SARSr-CoVs. Rs7327’s S protein varied from that of WIV1 and WIV16 at three aa residues in the receptor-binding motif, including one contact residue (aa 484) with human ACE2. This difference did not seem to affect its entry and replication efficiency in human ACE2-expressing cells. A previous study using the SARS-CoV infectious clone showed that the RsSHC014 S protein could efficiently utilize human ACE2 [33], despite being distinct from SARS-CoV and WIV1 in the RBD (S1 Fig). We examined the infectivity of Rs4231, which shared similar RBD sequence with RsSHC014 but had a distinct NTD sequence, and found the chimeric virus WIV1-Rs4231S also readily replicated in HeLa cells expressing human ACE2 molecule. The novel live SARSr-CoV we isolated in the current study (Rs4874) has an S gene almost identical to that of WIV16. As expected, it is also capable of utilizing human ACE2. These results indicate that diverse variants of SARSr-CoV S protein without deletions in their RBD are able to use human ACE2. In contrast, our previous study revealed that the S protein of a R. sinicus SARSr-CoV with deletions (Rp3) failed to use human, civet and bat ACE2 for cell entry [34]. In this study, in addition to Rs4231 and Rs7327, we also constructed infectious clones with the S gene of Rs4081, Rf4075, Rs4085, Rs4235 and As6526, which all contained the deletions in their RBD. These 7 strains, plus Rs4874 and the previously studied WIV1 and RsSHC014, could represent all types of S variants of SARSr-CoVs in this location (S3A Fig). However, none of the strains with deletions in the RBD could be rescued from Vero E6 cells. Therefore, the two distinct clades of SARSr-CoV S gene may represent the usage of different receptors in their bat hosts.” (p. 18 of 27)
Note 33: “Menachery VD, Yount BL Jr., Debbink K, Agnihothram S, Gralinski LE, Plante JA, et al. A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence”. Nat Med. 2015; 21: 1508±1513. https://doi.org/10.1038/nm.3985 PMID: 26552008
[See:
And,
North Carolina-Wuhan Coronavirus Creation & Galveston Lab Coronavirus Studies on Aging Mice and Men
]
“Author Contributions
Conceptualization: Lin-Fa Wang, Jie Cui, Zheng-Li Shi.
Data curation: Ben Hu.
Formal analysis: Ben Hu, Xing-Yi Ge, Jie Cui.
Funding acquisition: Peter Daszak, Lin-Fa Wang, Jie Cui, Zheng-Li Shi.
Investigation: Ben Hu, Lei-Ping Zeng, Xing-Lou Yang, Wei Zhang, Bei Li, Jia-Zheng Xie, Xu-Rui Shen, Ning Wang, Dong-Sheng Luo, Xiao-Shuang Zheng.
Methodology: Lei-Ping Zeng, Zheng-Li Shi.
Project administration: Zheng-Li Shi.
Resources: Xing-Lou Yang, Xing-Yi Ge, Yun-Zhi Zhang, Dong-Sheng Luo, Mei-Niang Wang.
Software: Jie Cui.
Supervision: Zheng-Li Shi.
Validation: Ben Hu.
Visualization: Ben Hu, Zheng-Li Shi.
Writing – original draft: Ben Hu.
Writing – review & editing: Peter Daszak, Lin-Fa Wang, Jie Cui, Zheng-Li Shi”
(p. 25 of 27)
Hu B, Zeng L-P, Yang X-L, Ge X-Y, Zhang W, Li B, et al. (2017) Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus. PLoS Pathog 13(11): e1006698. https://doi.org/10.1371/journal.ppat.1006698 https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006698
Peter Daszak and the Wuhan lab also got USAID funding, via a UC Davis program run by Mazet: Between 2009-2019, USAID “PREDICT”, headed by JK Mazet at UC-Davis, gave $1.1 million to Wuhan Institute of Virology via Daszak’s EcoHealth Alliance. NIAID funding to Wuhan Institute of Virology from FY 2014-2019 was $826,277. This, alone, totals $1,926,277. Why is no one calling out Mazet-UC Davis? https://miningawareness.wordpress.com/2021/06/11/usaid-predict-nih-gave-1-9-million-to-the-wuhan-wiv-lab-through-daszak-ecohealth-alliance-daszak-talks-china-partners-work-on-killer-viruses-biden-admin-plans/
Below are screen shots of the paper in case the original gets deleted from the web site. The pages referenced above were uploaded to show as larger. Click each image to enlarge size. Or see the pdf at https://doi.org/10.1371/journal.ppat.1006698
Hu B, Zeng L-P, Yang X-L, Ge X-Y, Zhang W, Li B, et al. (2017) Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus. PLoS Pathog 13(11): e1006698.
https://pubmed.ncbi.nlm.nih.gov/29190287/
Mining Awareness + 
You must be logged in to post a comment.